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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has been noticed in several tumors, which leads to chromosome instability, and so may well lead to tumorigenesis. This protein was discovered to affiliate with, and become associated with, the phosphorylation of NPAT protein (nuclear protein mapped towards the ATM locus), which participates in mobile-cycle regulated histone gene expression and plays a important position in marketing mobile-cycle progression within the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, happen to be explained. Two added splice variants were described but in depth nucleotide sequence information will not be however out there.
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Blocking peptides are peptides that bind specially to the goal antibody and block antibody binding. These peptide ordinarily is made up of the epitope regarded via the antibody.
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This guide outlines additional possible optimization of mobile and immunoassay parameters to make certain the best possible success are...
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This guideline outlines further possible optimization of cellular and immunoassay parameters to ensure the best possible results are...
Antibodies bound to the blocking peptide no longer bind to the epitope on the concentrate on protein. By evaluating the staining with the blocked antibody as opposed to the antibody on your own, one can see which staining is particular.
Target Information and facts Cyclin E belongs to your remarkably conserved cyclin household, whose associates are characterized by a extraordinary periodicity in protein abundance in the mobile cycle. Cyclins perform as regulators of CDK kinases. Various cyclins show unique expression and degradation patterns which add on the temporal coordination of every mitotic event. Cyclin E sorts a fancy with and functions for a regulatory subunit of CDK2, whose activity is necessary for mobile cycle G1/S changeover. This protein accumulates in the G1-S period boundary and it is degraded as cells development by means of S period.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both antibodies...
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